VEEV Marmoset Model
VEEV MARMOSET MODEL
Venezuelan equine encephalitis virus (VEEV) is a potentially re-emerging pathogen that has been responsible for several epizootics and epidemics. VEEV is also of interest due to its high infectivity when aerosolized, and its potential abuse as a bioterrorism or bio-warfare agent. IITRI has developed and characterize a marmoset model for aerosolized VEEV using an efficient bioaerosolization system for evaluation of therapeutics and vaccines. This VEEV marmoset model can be utilized to evaluate the efficacy of potential vaccines or antiviral therapeutic agents.
Marmosets were challenged with aerosolized VEEV via head-only aerosol exposure, with challenge dosages of 3.1 x 104 and 3.6 x 105 PFU. Animals were then monitored for weight loss, changes in body temperature and clinical scores for 14 days. Viral titers of blood collected on days 2, 4, 6 and 8 after challenge was determined by qPCR.
In the marmoset, the VEEV is infectious by aerosol exposure and leads to clinical disease, including loss of body weight and fever, hypothermia and hypoactivity. The VEEV disease that follows aerosol exposure in marmosets resembles that reported for human exposures to VEEV, making the marmoset a useful model for vaccine or therapeutic efficacy studies.
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