Mechanisms of Action

Mechanisms of action studies are used to identify the specific biochemical interactions in which a drug produces a pharmacological effect. A broader and deeper understanding of a drug candidate’s mechanism of action will enhance the design and interpretation of GLP safety and toxicology studies required for IND applications.

IITRI has broad capabilities in molecular biology and biochemistry to characterize the molecular targets and pathways that drug candidates interact with. Our experienced team of scientists can help you evaluate the mechanisms of actions associated with cancer biology and infectious disease, in addition to other therapeutic areas.

The IITRI Advantage

  • A highly published team of cancer biology scientists, with more than 250 publications in the field including mechanism of action
  • A highly experience team of infectious disease scientists from multiple disciplines including molecular biology, microbiology, virology, and immunology
  • A collaborative approach to study design for non-standard and customized studies

Core Services

Gene Expression Studies

  • Gene cloning
  • Gene knockdown analysis with siRNA technology
  • Inducible expression systems
  • Microarray analysis
  • miRNA analysis
  • Quantitative PCR analysis
  • Protein-protein interaction assays
  • Western blot analysis
  • Signal transduction pathway analysis

Cell-based Studies

  • Cell cycle analysis
  • Apoptosis measurements
  • Angiogenesis assays
  • Invasion assays
  • Cellular localization studies