BSL-3 Aerosol Challenge Studies
IITRI’s BSL-3 / ABSL-3 containment facility includes an aerosol generation apparatus for aerosol challenge studies with pathogens, select agents or toxins. Conducting aerosol challenge studies in a high-containment environment can be used for evaluation of medical countermeasures (MCMs) against aerosol-delivered infectious disease bioterrorism agents such as anthrax, F. tularensis, or Y. pestis, or for evaluation of treatments or vaccines towards aerosol-transmitted emerging diseases such as VEE, WEE, M. tuberculosis, and high-path influenza.
Aerosol generation systems for biological solutions (bacterial and viral cultures, proteins) must be designed to minimize destruction of bacterial and viral solutions, which are sensitive to the shear stress that is generated by aerosolization systems. IITRI utilizes a proprietary bioaerosol generation system that is comprised of six PARI LC plus nebulizers operated at lower pressures to minimize damage to microorganisms or biomolecules, and a 64-port nose-only exposure chamber through which supply and exhaust air are controlled by regulators and flow meters. Advantages of this system include:
|IITRI Inhalation System Feature||Advantage|
|Lower pressures for aerosol generation||Higher bacterial and viral survival rates|
|Shorter exposure times, higher spray factors|
|Nose only administration (rodents, rabbits)||Provides localized and quantifiable administration of test agent|
|Head-only administration (NHPs)||Quantifiable administration of test article along with real-time plethysmography|
|64-port inhalation exposure chamber||Allows testing a larger number of animals at once|
|Reduces number of exposure runs and decreases variability in the study|
|Efficient aerosol generation mechanism||Eliminates the need for concentration steps prior to aerosol generation|
|Reduced foaming incidence||Eliminates the need for surfactants that weaken bacterial cell walls|
|Animal models||Rodent, Rabbit, Non-human primate (NHP)|
Contact us to learn more about our bioaerosol generation capabilities.